HyaluronidasesareagroupofenzymesthatdegradeHyaluronan(HA),alinearpolysaccharidecomprisedofarepeatingdisaccharideofN-acetylglucosamineandD-glucuronicacid.HAisinvolvedinmanybiologicalprocessesincludingstructuralsupport,cellmigrationandtissueturnover.CirculatingHAlevelshavealsobeenshowntocorrelateinseveraldisorderssuchasliverdiseaseandcertaintypesofcancer.HyaluronidaseshavebecomeanimportantareaofstudyduetotheirregulatoryfunctioninHAmetabolismandtheirroleinphysiologicalprocessessuchasfertilizationandwoundhealing.ElevatedexpressionlevelsofthegeneencodingHyaluronidase1havebeenfoundinatypicalductalhyperplastictissues,suggestingthepossibilitythatitmaybeaviablebioMarkerforidentifyingpremalignantlesionsthatcandevelopintobreastcancer.Researchhasalsoshownthathyaluronidasesareinvolvedinseveralpathologicalprocessessuchasbacterialpathogenesis,thespreADIngoftoxins/venomsandcancerprogression,makinghyaluronidasesapotentialpharmacologicaltarget.

Echelon’sHyaluronidaseActivityELISAisaquantitativeimmunoassaydesignedforinvitromeasurementofhyaluronidaseactivityinbiologicalsamples.Hyaluronidasereactionsareperformedina96-wellmicrotiterplatepre-coatedwithHAsubstrate.TheactivityofthehyaluronidaseisdeterminedbycomparingHAsubstratelevelspostreactiontoastandardcurveofpre-coatedHAsubstrateamounts.Echelon’sHyaluronidaseActivityELISAhasbeenvalidatedwithhumansera,plasmaandurinesamplesinadditiontopurifiedhyaluronidasefrombovinetestes.Echelon’sHyaluronidaseActivityELISAprovidesarobustandsimplemethodforresearcherstomeasurehyaluronidaseactivityinbiologicalsamples.

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