ProductDescription:TheassayisacompetitiveELISAinwhichthesignalisinverselyproportionaltotheamountofPI(4,5)P2produced.OncePI(4,5)P2hasbeenextractedfromcellsamples,itisfirstincubatedwithaPI(4,5)P2detectorprotein,thenaddedtothePI(4,5)P2-coatedmicroplateforcompetitivebinding.Aperoxidase-linkedsecondarydetectionreagentandcolorimetricsubstrateisusedtodetectthePI(4,5)P2detectorproteinbindingtotheplate.ThecolorimetricsignalisinverselyproportionaltotheamountofPI(4,5)P2extractedfromcells.
Phosphatidylinositol4,5-bisphosphate,orPI(4,5)P2,isaubiquitouslipidshowntoplayacentralroleinavarietyofcellfunctionsincludingthePKB/AktpathwayleADIngtocellgrowth,motility,inflammation,andapoptosis.PI(4,5)P2isasubstrateforclassIphosphoinositide3-kinase(PI3-K)andtheproductofPTENphosphataseactivity.
FeaturedinPublications:
1.Ehrmantrout,KimberlyK.,Branch,AngieM.,Neilsen,PaulO.,andThompson,JamesE.."NonradioactiveQuantificationofPhosphoinositide3-Kinase(PI3K)ProductsfromBreastCancerCellLines".AmericanSocietyforCellBIOLOGy(ASCB),Washington,DC.2007.
2.Ehrmantrout,KimberlyK.,Branch,AngieM.,Neilsen,PaulO.."QuantificationofPI3-KinaseProductsfromBreastCancerCellLines".AmericanAssociationforCancerResearch(AACR).Denver,CO.2009.
3.Musse,A.A.,Gao,W.etal.“Myelinbasicproteinco-distributeswithotherPI(4,5)P2-sequesteringproteinsinTritonX-100detergent-resistantmembranemicrodomains”.NeuroscienceLetters,450(1):32-36
4.Costa,C.,Ebi,H.,etal."MeasurementofPIP3LevelsRevealsanUnexpectedRoleforp110betainEarlyAdaptiveResponsestop110alpha-SpecificInhibitorsinLuminalBreastCancer"Cell27(1):97-108
5.Gross,C.,C.-W.Chang,etal.(2015)."IncreasedExpressionofthePI3KEnhancerPIKEMediatesDeficitsinSynapticPlasticityandBehaviorinFragileXSyndrome."CellReports,DOI:10.1016/j.celrep.2015.03.060
6.S.Choi,etal."AgoNIST-stimulatedphosphatidylinositol-3,4,5-trisphosphategenerationbyscaffoldedphosphoinositidekinases"NatureCellBiology(2016)18(12),1324-35,doi:10.1038/ncb3441
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Keywords:PI3Kinase,PI3K,PIP2,PTEN,Phosphatidylinositol(4,5)-bisphosphate,PtdIns(4,5)P2,PI(4,5)P2,PIP2,Phosphatidylinositol3-kinase,PI3-kinase,PI3-Kinase,PI3-K,PI3K,PTEN,Phosphataseandtensinhomolog,Phosphatases,cellextraction,lipidextraction,extraction |
