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当前位置: 首页 > 产品中心 > Sandwich_method_ELISA > echelon inc/PI(4)P Mass ELISA试剂盒/1kit/K-4000E-1kit
商品详细echelon inc/PI(4)P Mass ELISA试剂盒/1kit/K-4000E-1kit
echelon inc/PI(4)P Mass ELISA试剂盒/1kit/K-4000E-1kit
echelon inc/PI(4)P Mass ELISA试剂盒/1kit/K-4000E-1kit
市场价: ¥11340.00
美元价: 5386.50
产地: 美国(厂家直采)
公司:
产品分类: 夹心法ELISA
公司分类: Sandwich_method_ELISA
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

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CatalogNo.: K-4000E

Description: Echelon'sPI(4)PMassELISAkitisdesignedtodetectandquantifyPI(4)PbymeansofacompetitiveELISAformat,eliminatingtheneedforrADIoactivityandthinlayerchromatography.ThePI(4)PMassELISAdirectlydetectsPI(4)PoverallotherphosphonositidesatrelevantBIOLOGicallevels.

AssayRange:50-0.7pmolPI(4)P
SampleType:Drieddownlipidextractedfromcells
SampleVolume:60μL/sample,singlepoint

ProductBackground:ThePI(4)PMassassayisacompetitiveELISAinwhichthesignalisinverselyproportionaltotheamountofPI(4)Pmeasured.ThePI(4)PextractionsareaddedtothecoloredmixingplatewithPI(4)PGrip.ThismixtureisthentransferredtothePI(4)PDetectionPlateforcompetitivebinding.Aperoxidase-linkedsecondarydetectorandcolorimetricdetectionisusedtodetecttheamountofPI(4)PGripbindingtotheplate.Theassayissensitiveto0.7pmolPI(4)P.

Storage

Thekitcomesintwopartswithdifferentstoragerequirements.UponreceiptstoreKitPart1at4°CandKitPart2at-20°C.Storepreparedreagentsasindicatedintheprotocol.

FeaturedinReferences

1)S.Choi,etal."AgoNIST-stimulatedphosphatidylinositol-3,4,5-trisphosphategenerationbyscaffoldedphosphoinositidekinases"NatureCellBiology(2016)18(12),1324-35,doi:10.1038/ncb3441

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品牌介绍
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