 | CatalogNo.: K-3200 Description: Echelon'sAcidSphingomyelinaseAssayKitisanenzymeassaythatmeasuresacidsphingomyelinaseactivityinBIOLOGicalsamplesthroughthedirecthydrolysisofafluorogenicsubstrate. Assaysensitivity:3.125pmol/hour SampleType:tissuehomogenates,celllysates SampleVolume:20uL/sample(forduplicatepoints) | ||
ProductBackground:Sphingomyelinasescatalyzesthehydrolysisofsphingomyelinintoceramideandphosphorylcholine;andisinvolvedinprogrammedcelldeath(apoptosis),celldifferentiationandcellproliferation.Sphingomyelinasesareclassifiedintofivecategories:acidsphingomyelinase(aSMase),secretorysphingomyelinase(sSMase),neutralMg2+/-dependentsphingomyelinases(nSMase),neutralMg2+/-independentsphingomyelinasesandalkalinesphingomyelinase.Acidsphingomyelinasewasthefirstdescribedandbestcharacterizedofthesphingomyelinases.Adeficiencyoflysosomalacidsphingomyelinaseleadstorapidneurodegenerationanddeathduetoexcessiveaccumulationofsphingomyelin(Niemann-Pickdisease). | |||
