 | CatalogNo.: K-1800 Description: Echelon’sNeutralSphingomyelinase(N-SMase)AssaykitisanenzymecoupledassaythatmeasuressphingomyelinaseactivityinBIOLOGicalsamplesthroughthedownstreamproductionofcholine. AssayIncubationtime:2-4hours AssayRange:5mU/mL-0.078mU/mL SampleType:tissuehomogenates,celllysates,serum,saliva,andurine | ||
ProductBackground:Inthisenzymecoupledassay,NeutralSphingomyelinase(N-SMase)catalyzesthehydrolysisofsphingomyelinintophosphorylcholineandceramide.Alkalinephosphatasethencatalyzesphosphorylcholinetocholine.CholineisthemoxidizedbyCholineoxidaetoformhydrogenperoxide.Finally,DAOSplus4-Aminoantipyrine(4AAP)inthepresenceofhydrogenperoxideandperoxidaseresultsintheoxidativecouplingofDAOSand4AAPtoformabluechromogenthatisdetectedbymeasuringtheabsorbanceoflightat595nm. | |||
