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当前位置: 首页 > 产品中心 > Sandwich_method_ELISA > echelon inc/PI(5)P4激酶(PIP4KII)活性ELISA/1kit/K-5400-1kit
商品详细echelon inc/PI(5)P4激酶(PIP4KII)活性ELISA/1kit/K-5400-1kit
echelon inc/PI(5)P4激酶(PIP4KII)活性ELISA/1kit/K-5400-1kit
echelon inc/PI(5)P4激酶(PIP4KII)活性ELISA/1kit/K-5400-1kit
商品编号: K-5400-1kit
市场价: ¥11900.00
美元价: 5652.50
产地: 美国(厂家直采)
公司:
产品分类: 夹心法ELISA
公司分类: Sandwich_method_ELISA
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

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CatalogNo.: K-5400

Description: Echelon’sPI(5)P4-KinaseActivityAssayisanATPdepletionassaywhichquantifiestheremainingATPlevelsinsolutionfollowingthekinasereaction.TheassaycanbeusedtoquantifyPI(5)P4-Kinaseinvitroorincelllysate.



TypeIIPI(5)P4-Kinase(PIP4KII)catalyzesthephosphorylationofphosphatidylinositol-5-phosphate[PI(5)P]tophosphatidylinositol-4,5-bisphosphate[PI(4,5)P2].PIP4KIIhasbeenshowntoplayaroleincertaintypesofbreastandcoloncancerinadditiontobeingimportantinseveralphysiologicalprocessessuchasinsulinsignaling,stressresponse,andembryonicdevelopmentinseveralmodelorganisms.IthasalsobeenimplicatedasanimportantregulatorofnuclearPI(5)Plevels.
TheassayreactionispreparedbytheadditionofPIP4KII(enzyme),PI(5)P(substrate)inreactionbufferandinitiatedbytheadditionofATP.ThereactionisthenstoppedbyaddingtheATPdetectorafterthechosenreactiontime.Theluminescentsignalisinverselycorrelatedwiththekinaseactivity.Echelon’sPI(5)P4-KinaseActivityAssayistestedusingSignalchem’sPIP4KIIA(P76-10AG)kinase.

Storage

-20°C

RelatedProducts:

Substrates:P-5016,P-5008
RelatedEnzymes:PI3Kα(E-2000),PTEN(E-3000),SHIP2(E-1000)
RelatedAssays:PI(4)P5-Kinase(PIP4KI)ActivityAssay(K-5700),PI3-KinaseActivityELISA(K-1000s),PTENActivityELISA(K-4700)
品牌介绍
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