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CatalogNo.: T-1300a
Description: ThePI3KInhibitorScreeningServiceisacompetitiveassayfortheproductofthePI3-Kinasereaction,PI(3,4,5)P3.Theenzymeusedintheserviceispurifiedrecombinanthumanp110α/p85αactiveenzymeandthesubstrateisdiC8PI(4,5)P2.DuplicatePI3Kreactionsareperformedinthepresenceofeachsamplecompoundprovidedbythecustomer.AfterthePI3Kreactionsarecomplete,reactionproductsaredetectedusingLuminescentAssayforPI3-KinaseActivity(AlphaScreen®Assay,cat#K-1300).
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Afulltestserviceisdividedintotwoparts-(Stage1)screeningforpotentialinhibitorstargetinghumanPI3Kp110α/p85α,and(Stage2)determinationofIC50valueforhitcompoundfromstage1.Whentestingforinhibitionpotentialinstage1,asingleselectedcompoundconcentration(determinedbycustomer,usuallyahighdose)forallcompoundsampleswillbetested.Assaycontrolsinclude“PositiveReactioncontrol”withoutcompoundorinhibitor;“PositiveInhibitioncontrols”withawellknownPI3Kinhibitor;“Substratecontrol”withoutenzymeandinhibitor;and“CompoundInterferencecontrol”withoutenzyme.API(3,4,5)P3Standardcurvecanbeincludeduponrequestwithextracharge.Whenasamplecompoundshowsinhibitionpotential(ahit)andfurthercharacterizationisrequired,IC50valueofthecompoundcanbedeterminedthroughcompoundtitrationastestservicestage2.
Pleaserefertothetechnicaldatasheetforsampleconsiderationsandshippinginformation.ToplaceanorderpleasecallEchelonat801.588.0455
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