Lipid coated beads are designed for use in protein pull-down experiments. Use to test for lipid binding of purified protein, proteins in cell lysate, or binding of radiolabeled in vitro translation products.

PI(4,5)P2 Beads are composed of agarose with 10 nanomoles of bound PI(4,5)P2 per mL of beads, enough for several protein binding experiments. Each 1 mL order of Beads includes a small amount of control beads. Larger quantities of Control Beads are also available for purchase.

Storage

4 °C – do not freeze

1) Gupta, S., J. C. Fanzo, et al. (2003). “T cell receptor engagement leads to the recruitment of IBP, a novel guanine nucleotide exchange factor, to the immunological synapse.” J Biol Chem 278(44): 43541-9.2) Kawahara, T. and J. D. Lambeth (2008). “Phosphatidylinositol (4,5)-bisphosphate modulates Nox5 localization via an N-terminal polybasic region.” Mol Biol Cell 19(10): 4020-31.3) Kim, S. K., H. Kim, et al. (2011). “Phosphatidylinositol phosphates directly bind to neurofilament light chain (NF-L) for the regulation of NF-L self assembly.” Exp Mol Med 43(3): 153-60.4) Lewis, A. E., et al. (2011). “Identification of nuclear phosphatidylinositol 4,5-bisphosphate-interacting proteins by neomycin extraction.” Mol Cell Proteomics 10(2): M110 003376.5) Ulicna, L., et al. (2018). “PIP2 epigenetically represses rRNA genes transcription interacting with PHF8.” Biochimica et Biophysica Acta (BBA) – Molecular and Cell Biology of Lipids 1863(3): 266-275.6) Huang, D., et al. (2019). “Hypoxia induces actin cytoskeleton remodeling by regulating the binding of CAPZA1 to F-actin via PIP2 to drive EMT in hepatocellular carcinoma.” Cancer Letters 448(28): 117-127.

Being bio-reactive blog: “Lipid-Ligand interaction tools: lipids are not scary intermediates anymore”

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