Z-PETH reacts primarily with phosphatidylethanol (of synthetic or natural origin), and demonstrates low cross-reactivity with other lipids and phospholipids including phosphatidylcholine, phosphatidylethanolamine, phosphatidic acid, phosphatidylserine, phosphatidylglycerol, sphingomyelin, andcholesterol.

The following applications have been demonstrated by Echelon Biosciences: ELISA (0.5 μg/mL) and fluorescent microscopy (2.5 μg/mL). Z-PETH recognizes phosphatidylethanol (PEth), biotinylated PEth, or fluorescent PEth depending on the application. Additional in vitro and cellular applications are possible using this antibody, but have not been verified by Echelon.

Storage

4 °C for up to 30 days. Aliquot and store at -20 or -80 °C if longer storage is necessary. Avoid repeated freeze/thaw cycles.

Featured in Publications

1) Esteban-Pretel, G., M. P. Marin, et al. (2012). “Polyphosphoinositide Metabolism and Golgi Complex Morphology in Hippocampal Neurons in Primary Culture is Altered by Chronic Ethanol Exposure.” Alcohol and Alcoholism.