CAS Number: 149231-66-3Molecular Weight: 628.25Salt Form: N/APurity: >96%Sequence (3-letter): Ac-Tyr-Val-Ala-Asp-pNASequence (1-letter): Ac-YVAD-pNAStorage: -20 °C or belowSolubility: DMSO to 10 mM; soluble in aqueous base (e.g. 0.1 N NH4OH) to 1 mg/mL

Caspase 1 (ICE) Substrate (Ac-Tyr-Val-Ala-Asp-pNA) is a substrate for interleukin 1b converting enzyme (ICE) which is a cysteine protease that cleaves specifically at aspartic acid. ICE converts inactive IL-1 beta precursor to a pro-inflammatory cytokine that mediates a wide range of immune and inflammatory response. ICE-related cysteine proteases are required for E1A-induced, p53-dependent apoptosis in baby rat kidney (BRK) cells. Adenovirus E1B 19K protein, which is a potent inhibitor of apoptosis, inhibits activation of these proteases in BRK cellsKm = 23 μM (Casp-1), 29,000 μm (Casp-3), 874 μM (Casp-4)

Activity is quantified by the release of p-nitroaniline which is measured by absorbance at 405 nm.

References

1. Alcamí A, Smith GL. A soluble receptor for interleukin-1 beta encoded by vaccinia virus: a novel mechanism of virus modulation of the host response to infection. Cell. 1992 71 (1): 153–167.2.Ayala JM, Yamin TT, Egger LA, Chin J, Kostura MJ, Miller DK. IL-1 beta-converting enzyme is present in monocytic cells as an inactive 45-kDa precursor. J Immunol. 1994 Sep 15;153(6):2592–2599. 3.Kuida, K. et al (1995) “Altered cytokine export and apoptosis in mice deficient in interleukin-1 beta converting enzyme” Science 267 (5206): 2000-2003.