4000-520-616
欢迎来到免疫在线!(蚂蚁淘生物旗下平台)  请登录 |  免费注册 |  询价篮
主营:Echelon提供各种抗体、分析检测试剂盒和抑制剂等产品,尤其擅长以细胞信号、脂类代谢和标记物研究为主的相关产品。为研究治疗诸如癌症、糖尿病、炎症、感染和心脑血管疾病专家提供全面有效工具。
咨询热线电话
4000-520-616
当前位置: 首页 > 产品中心 > Other_kits > Echelon/PTEN Activity ELISA Kit/EA KIT/K-4700
商品详细Echelon/PTEN Activity ELISA Kit/EA KIT/K-4700
Echelon/PTEN Activity ELISA Kit/EA KIT/K-4700
Echelon/PTEN Activity ELISA Kit/EA KIT/K-4700
商品编号: K-4700
市场价: ¥15340.00
美元价: 7286.50
产地: 美国(厂家直采)
公司:
产品分类: 其它检测试剂盒
公司分类: Other_kits
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

The PTEN Activity ELISA is designed to quantify the phosphatase activity of PTEN by detection of the enzyme product, PI(4,5)P2.


Sample Type: Recombinant or immunoprecipitated PTEN enzyme (IP protocol at end of Technical Data Sheet)Assay Range: 12.5 to 200 pmol PI(4,5)P2


Product BackgroundThe PTEN Activity ELISA directly detects the phosphoinositide product. Other assays detect free phosphate. This eliminates many possible sources of error due to the fact that inorganic phosphate is the product of many phosphatase enzyme activities, and is found in common buffers and cleaning products.

The assay is a competitive ELISA in which the signal is inversely proportional to the amount of PI(4,5)P2 produced. After the PTEN reactions are complete, reaction products are added to the PI(4,5)P2-coated microplate and a PI(4,5)P2 detector protein is added for competitive binding. A peroxidase-linked secondary detector and colorimetric detection is used to detect PI(4,5)P2 detector binding to the plate. The colorimetric signal is inversely proportional to the amount of PI(4,5)P2 produced by PTEN.

PTEN (Phosphatase and Tensin Homolog deleted on Chromosome 10) is a 3’ phosphoinositide phosphatase that converts PI(3,4,5)P3 to PI(4,5)P2, thus opposing PKB/Akt activation by PI 3-K. PTEN is involved in neuronal stem cell proliferation and self-renewal, cardiac myocyte hypertrophy and contractility, and a wide range of developmental processes. PTEN, however, is best known for its role as a tumor suppressor. Loss of PTEN activity results in accumulation of PI(3,4,5)P3, abnormal activation of PKB/Akt, unregulated cell growth, suppression of apoptosis, and increased tumorigenesis in a number of human tissues. It has also been proposed that PTEN is a candidate for targeted chemotherapy because certain anti-cancer agents preferentially destroy tumors with PTEN mutations. In addition to this direct role in cancer, PTEN has recently been shown to regulate cancer-associated pathways including VEGF-mediated angiogenesis and others.

Categories

Kits & Assays

Filter

Activity, ELISA, PTEN

品牌介绍
Echelon产品线★合成磷脂、磷酸肌醇、鞘脂及其衍生物★脂蛋白质相互作用工具★脂质含量检测试剂盒★脂质相关生物酶活性检测,抑制剂筛选试剂盒