The PI(4)P Mass ELISA Kit is designed to detect and quantify PI(4)P by means of a competitive ELISA format.

Assay Range: 50-0.7 pmol PI(4)PSample Type: Dried down lipid extracted from cellsSample Volume: 60 µL/sample, single point

Product BackgroundThe PI(4)P Mass ELISA Kit is designed to detect and quantify PI(4)P by means of a competitive ELISA format, eliminating the need for radioactivity and thin layer chromatography. The PI(4)P Mass ELISA directly detects PI(4)P over all other phosphonositides at relevant biological levels.

The PI(4)P Mass ELISA Kit is a competitive ELISA in which the signal is inversely proportional to the amount of PI(4)P measured. The PI(4)P extractions are added to the colored mixing plate with PI(4)P Grip. This mixture is then transferred to the PI(4)P Detection Plate for competitive binding. A peroxidase-linked secondary detector and colorimetric detection is used to detect the amount of PI(4)P Grip binding to the plate. The assay is sensitive to 0.7 pmol PI(4)P.